在藥品研發(fā)中，大家肯定遇到在復(fù)核USP標(biāo)準(zhǔn)的時(shí)候，總為不同的色譜柱而發(fā)愁，常常不能準(zhǔn)確的確定USP規(guī)定的色譜柱，以及與我們常用色譜柱的關(guān)系，本文主要?dú)w納一下USP色譜柱與我們常用色譜柱的關(guān)系。

 

L1和L8是美國(guó)藥典(USP)規(guī)定的色譜柱編號(hào)，其實(shí)就是ODS柱和NH2柱。下面是USP規(guī)定的編號(hào)所對(duì)應(yīng)的色譜柱類(lèi)型。

 

L1：十八烷基鍵合多孔硅膠或無(wú)機(jī)氧化物微粒固定相，簡(jiǎn)稱(chēng)ODS柱

 

L2：30～50mm表面多孔薄殼型鍵合十八烷基固定相，簡(jiǎn)稱(chēng)C18柱

 

L3：多孔硅膠微粒，即一般的硅膠柱

 

L4：30～50mm表面多孔薄殼型硅膠柱

 

L5：30～50mm表面多孔薄殼型氧化鋁柱

 

L6：30～50mm實(shí)心微球表面包覆磺化碳氟聚合物，強(qiáng)陽(yáng)離子交換柱

 

L7：全多孔硅膠微粒鍵合C8官能團(tuán)固定相，簡(jiǎn)稱(chēng)C8柱

 

L8：全多孔硅膠微粒鍵合非交聯(lián)NH2固定相，簡(jiǎn)稱(chēng)NH2柱

 

L9：強(qiáng)酸性陽(yáng)離子交換基團(tuán)鍵合全多孔不規(guī)則形硅膠固定相，即SCX柱

 

L10：多孔硅膠微球鍵合氰基固定相（CN），簡(jiǎn)稱(chēng)CN柱

 

L11：鍵合苯基多孔硅膠微球固定相，簡(jiǎn)稱(chēng)苯基柱

 

L12：無(wú)孔微球鍵合季胺功能團(tuán)的強(qiáng)陰離子交換柱

 

L13：三乙基硅烷化學(xué)鍵合全多孔硅膠微球固定相（C1），簡(jiǎn)稱(chēng)C1柱

 

L14：10mm硅膠化學(xué)鍵合強(qiáng)堿性季銨鹽陰離子交換固定相，簡(jiǎn)稱(chēng)SAX柱

 

L15：已基硅烷化學(xué)鍵合全多孔硅膠微球固定相，簡(jiǎn)稱(chēng)C6柱

 

L16：二甲基硅烷化學(xué)鍵合全多孔硅膠微粒固定相 C2柱

 

L17：氫型磺化交聯(lián)苯乙烯-二乙烯基苯共聚物,強(qiáng)陽(yáng)離子交換柱

 

L18：3～10mm全多孔硅膠化學(xué)鍵合胺基（NH2）和氰基（CN）柱

 

L19：鈣型磺化交聯(lián)苯乙烯－二乙烯基苯共聚物，強(qiáng)陽(yáng)離子交換柱

 

L20：二羥基丙烷基化學(xué)鍵合多孔硅膠微球固定相（Diol），簡(jiǎn)稱(chēng)二醇基柱

 

L21：剛性苯乙烯－二乙烯基苯共聚物微球填料柱

 

L22：帶有磺酸基團(tuán)的多孔苯乙烯陽(yáng)離子交換柱

 

L23：帶有季胺基團(tuán)的聚甲基丙烯酸甲酯或聚丙烯酸酯多孔離子交換柱

 

L24：表面含有大量羥基的半剛性聚乙烯醇親水凝膠柱

 

L25：聚甲基丙烯酸酯樹(shù)脂交聯(lián)羥基醚（表面含有殘余羧基功能團(tuán)）樹(shù)脂。能分離分子量100～5000MW范圍的水溶性中性、陽(yáng)離子型及陰離子型聚合物（用聚氧乙烯測(cè)定）的固定相

 

L26：丁基硅烷化學(xué)鍵合全多孔硅膠微球固定相，即C4柱

 

L27：30～50mm的全多孔硅膠微粒

 

L28：多功能載體，100Å的高純硅膠加以氨基鍵合以及C8反相鍵合的官能團(tuán)

 

L29:氧化鋁,反相鍵合,含碳量低,氧化鋁基聚丁二稀小球,5mm，孔徑80Å

 

L30:全多孔硅膠鍵合乙基硅烷固定相

 

L31:季胺基改性孔徑2000Å的交聯(lián)苯乙烯和二乙烯基苯(55%)強(qiáng)陰離子交換樹(shù)脂

 

L32: L-脯氨酸銅配合物共價(jià)鍵合于不規(guī)則形硅膠微粒的配位體的交換手性色譜填料

 

L33:能夠分離分子量4000～40000MW范圍蛋白質(zhì)分子的球形硅膠固定相, pH穩(wěn)定性好

 

L34：鉛型磺化交聯(lián)苯乙烯－二乙烯基苯共聚物強(qiáng)陽(yáng)離子交換樹(shù)脂，9mm球形

 

L35：鋯穩(wěn)定的硅膠微球鍵合二醇基親水分子單層固定相，孔徑150Å

 

L36:5mm胺丙基硅膠鍵合L-苯基氨基乙酸－3,5二硝基苯甲酰

 

L37：適合分離分子量2000～40000MW的聚甲基丙烯酸酯凝膠

 

L38：水溶性甲基丙烯酸酯基質(zhì)SEC色譜柱

 

L39：親水全多孔聚羥基甲基丙烯酸酯色譜柱

 

L40：Tris 3,5－二甲基苯基氨基甲酸酯纖維素涂覆多孔硅膠微球

 

L41：球形硅膠表面固定α1酸糖蛋白固定相

 

L42: C8和C18硅烷化學(xué)鍵合多孔硅膠固定相

 

L43:硅膠微球鍵合五氟代苯基固定相

 

L44:多功能固定相,60 Å高純硅膠基質(zhì)鍵合磺酸陽(yáng)離子交換功能團(tuán)和C8反相功能團(tuán)

 

L45: β-環(huán)糊精鍵合多孔硅膠微球

 

L46:季胺基改性苯乙烯-二乙烯基苯聚合物微球

 

L1 Octadecyl silane chemically bonded to porous silica orceramic.

 

L1 十八烷基鍵合硅烷化學(xué)鍵合于多孔硅膠或陶瓷微粒，3-10u。

 

L2 Octadecyl silane chemically bonded to silica gel of acontrolled surface porosity that has been bonded to a solid spherical core, 30to 50 μm in diameter.

 

L2 十八烷基鍵合硅烷化學(xué)鍵合于表面空隙率一定的鍵合在緊密球核上的硅膠，粒徑30-50u。

 

L3 Porous silica particles, 5 to 10 μm in diameter.

 

L3 多孔硅膠顆粒，粒徑5-10u。

 

L4 Silica gel of controlled surface porosity bonded to asolid spherical core,30 to 50 μm in diameter.

 

L4 表面空隙率一定的硅膠鍵合于緊密球核上，粒徑30-50u。

 

L5 Alumina of controlled surface porosity bonded to asolid spherical core,30 to 50 μm in diameter.

 

L5 表面空隙率一定的氧化鋁鍵合于緊密球核上，粒徑30-50u。

 

L6 Strong cation-exchange packing: sulfonatedfluorocarbon polymer coated on a solid spherical core, 30 to 50 μm in diameter.

 

L6 強(qiáng)陽(yáng)離子交換填料：磺化氟代烴聚合物涂漬于緊密球核上，粒徑30-50u。

 

L7 Octyl silane chemically bonded to totally poroussilica particles, 1.5 to 10 μm in diameter.

 

L7 辛基硅烷化學(xué)鍵合于完全多孔硅膠擔(dān)體，粒徑10u。

 

L8 An essentially monomolecular layer ofaminopropyl-silane chemically bonded  to totally porous silica gelsupport, 3 to 10 μm in diameter.

 

L8 氨丙基硅烷單分子層化學(xué)鍵合于完全多孔硅膠擔(dān)體，粒徑10u。

 

L9 Irregular or spherical, totally porous silica gelhaving a chemically bonded, strongly acidic ation-exchange coating, 3 to 10 μmin diameter.

 

L9 10u不規(guī)則的完全多孔硅膠具有化學(xué)鍵合的強(qiáng)酸性陽(yáng)離子交換涂層。

 

L10 Nitrile groups chemically bonded to porous silicaparticles, 3 to 10 μm in diameter.

 

L10 氰基團(tuán)化學(xué)鍵合于多孔微粒硅，粒徑3-10u。

 

L11 Phenyl groups chemically bonded to porous silicaparticles, 1.5 to 10 μm in diameter.

 

L11 苯基化學(xué)鍵合于多孔微粒硅，粒徑3-10u。

 

L12 Strong anion-exchange packing made by chemically bondinga quaternary amine to a solid silica spherical core, 30 to 50 μm in diameter

 

L12 由四元胺化學(xué)鍵合于精密硅球核形成的強(qiáng)陰離子交換填料，粒徑30-50u。

 

L13 Trimethylsilane chemically bonded to porous silicaparticles,3 to 10 μm in diameter.

 

L13 三甲基硅烷化學(xué)鍵合于完全多孔硅膠，粒徑3-10u。

 

L14 Silica gel having a chemically bonded, strongly basicquaternary ammonium anion-exchange coating, 5 to 10 μm in diameter.

 

L14 具有強(qiáng)堿性四元胺陰離子交換涂層的10u硅膠化學(xué)鍵合相。

 

L15 Hexyl silane chemically bonded to totally poroussilica particles,3 to 10 μm in diameter.

 

L15 己基硅烷化學(xué)鍵合于完全多孔性微粒硅，粒徑3-10u。

 

L16 Dimethyl silane chemically bonded to totally poroussilica particles, 5 to 10 μm in diameter.

 

L16 二甲基硅烷化學(xué)鍵合于完全多孔性微粒硅，粒徑3-10u。

 

L17 Strong cation-exchange resin consisting of sulfonatedcross-linked styrene-divinylbenzene copolymer in the hydrogen form, 7 to 11 μmin diameter.

 

L17 由磺化的苯乙烯-二乙烯基苯交連共聚物組成的氫型陽(yáng)離子交換樹(shù)脂，粒徑7-11u。

 

L18 Amino and cyano groups chemically bonded to poroussilica particles,3 to 10 μm in diameter.

 

L18 氨基和氰基化學(xué)鍵合于多孔微粒硅，粒徑3-10u。

 

L19 Strong cation-exchange resin consisting of sulfonatedcross-linked styrene-divinylbenzene copolymer in the calcium form, 9 μm indiameter.

 

L19 由磺化的苯乙烯-二乙烯基苯交連共聚物組成的鈣型陽(yáng)離子交換樹(shù)脂，粒徑7-11u。

 

L20 Dihydroxypropane groups chemically bonded to poroussilica particles, 5 to 10 μm in diameter.

 

L20 二甲氧基丙烷基化學(xué)鍵合于多孔硅膠微粒，粒徑5-10u。

 

L21 Arigid, spherical styrene-divinylbenzene copolymer, 5 to 10 μm in diameter.

 

L21 剛性球形苯乙烯-二乙烯基苯共聚物，粒徑5-10u。

 

L22 Acation exchange resin made of porous polystyrene gel with sulfonic acid groups,about 10 μm in size.

 

L22 由多孔聚苯乙烯凝膠和磺酸基組成的陽(yáng)離子交換樹(shù)脂，粒徑約為10u。

 

L23 An anion exchange resin made of porouspolymethacrylate or polyacrylate gel with quaternary ammonium groups, about 10μm in size.

 

L23 由多孔聚甲基丙烯酸酯或聚丙烯酸酯凝膠及四元胺基組成的陰離子交換樹(shù)脂，粒徑約為10u。

 

L24 Asemi-rigid hydrophilic gel consisting of vinyl polymers with numerous hydroxylgroups on the matrix surface, 32 to 63 μm in diameter.

 

L24 由乙烯基聚合物和基質(zhì)表面的無(wú)數(shù)羥基組成的半剛性親水硅膠，粒徑32u-63u。

 

L25 Packing having the capacity to separate compoundswith a MW range from 100 to 5000 daltons (as determined by polyethylene oxide),applied to neutral, anionic, and cationic water-soluble polymers. Apolymethacrylate resin base,crosslinked with poly-hydroxylated ether (surfacecontained some residual carboxyl functional groups) was found suitable.

 

L25 能分離MW從100到5000Da化合物的填料（取決于聚環(huán)氧乙烷），應(yīng)用于中性、陰離子和陽(yáng)離子的水溶性聚合物。聚甲基丙烯酸酯作為樹(shù)脂基質(zhì)，與聚羥基化醚（表面含一些殘余羧基官能團(tuán)）進(jìn)行交連。

L26 Butyl silane chemically bonded to totally poroussilica particles, 5 to 10 μm in diameter.

 

L27 Porous silica particles, 30 to 50 μm in diameter.

 

L28 Amultifunctional support, which consists of a high purity, 100 , sphericalsilica substrate that has been bonded with anionic (amine) functionality inaddition to a conventional reversed phase C8 functionality.

 

L29 Gamma alumina, reversed phase, low carbon percentageby weight, alumina-based polybutadiene spherical particles, 5 μm diameter witha pore diameter of 80 .

 

L30 Ethyl silane chemically bonded to a totally poroussilica particle, 3 to 10 μm in diameter.

 

L31 Astrong anion-exchange resin-quaternary amine bonded on latex particles attachedto a core of 8.5 μm macroporous particles having a pore size of 2000 Å

 

and consisting of ethylvinylbenzene cross-linked with 55% divinyl benzene.

 

L32 Achiral ligand-exchange packing- L-proline copper complex covalently bonded toirregularly shaped silica particles, 5 to 10 μm in diameter.

 

L33 Packing having the capacity to separate proteins of4,000 to 400,000 daltons. It is spherical, silica-based and processed toprovide pH stability.

 

L34 Strong cation-exchange resin consisting of sulfonatedcross-linked styrene-divinylbenzene copolymer in the lead form, about 9 μm indiameter.

 

L35 Azirconium-stabilized spherical silica packing with a hydrophilic (diol-type)molecular monolayer bonded phase having a pore size of 150 .

 

L36 3,5-dinitrobenzoyl derivative of L-phenylglycinecovalently bonded to 5 μm aminopropyl silica.

 

L37 Polymethacrylate gel packing having the capacity toseparate proteins by molecular size over a range of 2,000 to 40,000D.

 

L38 Methacrylate-based size-exclusion packing forwater-soluble samples.

 

L39 Hydrophilic polyhydroxymethacrylate gel of totallyporous spherical resin.

 

L40 Cellulose tris-3,5-dimethylphenylcarbamate coatedporous silica particles,5 μm to 20 μm in diameter.

 

L41 Immobilized á-acid glycoprotein on spherical silicaparticles, 5 μm in diameter.

 

L42 Octylsilane and octadecylsilane groups chemicallybonded to porous silica particles,5 μm in diameter.

 

L43 Pentafluorophenyl groups chemically bonded to silicaparticles, 5 to 10 μm in diameter.

 

L44 Amultifunctional support, which consists of a high purity, 60 , spherical silicasubstrate that has been bonded with a cationic exchanger, sulfonic acidfunctionality in addition to a conventional reversed phase C8 functionality.

 

L45 Beta cyclodextrin bonded to porous silica particles,5 to 10 μm in diameter.

 

L46 Polystyrene/divinylbenzene substrate agglomeratedwith quaternary amine functionalized latex beads, 10 μm in diameter.

 

L47 High capacity anion-exchange microporous substrate,fully functionalized with a trimethylamine group, 8 μm in diameter.

 

L48 Sulfonated, cross-linked polystyrene with an outerlayer of submicron, porous,anion-exchange microbeads, 15 μm in diameter.

 

L49 Areversed-phase packing made by coating a thin layer of polybutadiene on tospherical porous zirconia particles, 3 to 10 μm in diameter.

 

L50 Multifunction resin with reversed-phase retention andstrong anion-exchange functionalities. The resin consists of ethylvinylbenzene,55 % cross-linked with

 

divinylbenzene copolymer, 3 to 15 μm in diameter, and asurface area of not less than 350 m2/g, substrate is coated with quaternary ammoniumfunctionalized latex particles consisting of styrene cross-linked withdivinylbenzene.

 

L51 Amylose tris-3,5-dimethylphenylcarbamate-coated,porous, spherical, silica particles,

 

5 to 10 μm in diameter.

 

L52 Astrong cation exchange resin made of porous silica with sulfopropyl groups, 5to 10 μm in diameter.

 

L53 Weak cation-exchange resin consisting ofethylvinylbenzene, 55 % cross-linked with

 

divinylbenzene copolymer, 3 to 15 μm diameter. Substrateis surface grafted with carboxylic acid and/or phosphoric acid functionalizedmonomers. Capacity not less than 500 μm in diameter.

 

L54 Asize exclusion medium made of covalent bonding of dextran to highlycross-linked porous agarose beads, about 13 μm in diameter.

 

L55 Astrong cation exchange resin made of porous silica coated withpolybutadiene-maleic acid copolymer, about 5 μm in diameter.

 

L56 Isopropyl silane chemically bonded to totally poroussilica particles, 3 to 10 μm in diameter.

 

L57 A chiral-recognitionprotein, ovomucoid, chemically bonded to silica particles, about 5 μm indiameter, with a pore size of 120 angstroms.

 

L58 Strong cation-exchange resin consisting of sulfonatedcross-linked styrene- divinylbenzene copolymer in the sodium form, about 7 to11 μm diameter.

 

L59 To separate proteins by molecular weight over therange of 10 to 500 kDa. Spherical 10 μm, silica-based, and processed to providehydrophilic characteristics and pH stability.

 

L60 Spherical, porous silica gel, 3 to 10 μm in diameter,surface has been covalently modified with palmitamidopropyl groups andendcapped.

 

L61 Hydroxide-selective, strong anion-exchange resinconsisting of a highly cross-linked core of 13 μm microporous particles, poresize less than 10 , and consisting of ethylvinylbenzene cross-linked with 55 %divinylbenzene with a latex coating

 

composed of 85 nm diameter microbeads bonded with alkanolquarternary ammonium ions (6 %).

 

L62 C30silane bonded phase on a fully porous spherical silica, 3 to 15 μm in diameter.